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Method and apparatus for cryopreserving biological

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专利名称:Method and apparatus for cryopreserving

biological specimens

发明人:Visinoni, Francesco,Bellini, Marco,Bellini,

Michele

申请号:EP14193239.2申请日:20141114公开号:EP3020276B1公开日:20180228

摘要:The present invention relates to a method for collecting and preservingbiological specimens for biobanking cryopreservation, comprising the steps of i) placingthe specimen in a cooling fluid pre-set at a first predetermined temperature of between -10°C and -60°C for snap freezing the specimen, and ii) reducing the temperature of saidcooling fluid to a second predetermined temperature suitable for preserving saidspecimen. The second temperature is most preferably -80°C. The reduction of thetemperature in step ii) is performed by a predetermined cooling profile. The invention isfurther directed to a corresponding apparatus (1), comprising a receiving means (2) forreceiving a cooling fluid and the specimen, a cooling means to cool said fluid in saidreceiving means, and a controller configured to control the cooling means. The receivingmeans comprises at least one reservoir (3, 30) and a working plate (4).The cooling meansis preferably a stirling cooler using Argon gas.

申请人:MILESTONE SRL

地址:IT

国籍:IT

代理机构:Kiwit, Benedikt

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