专利名称：PROCESS FOR THE QUANTITATIVE
DETERMINATION OF THE COPIER NUMBEROF A PREDETERMINED SEQUENCE IN ASAMPLE
发明人：MANN, WOLFGANG申请号：EP09733308.2申请日：20090415公开号：EP2315854A1公开日：20110504
摘要：A process for the quantitative determination of the number of at least onepredetermined sequence in a biological sample comprises the steps: a) providing abiological sample which contains a nucleic acid, b) fragmenting the nucleic acid present inthe biological sample, c) dividing the sample obtained in step b) into at least twosubsamples, d) adding in each case at least two primer pairs to each of the at least twosubsamples, where in each case the same primer pairs are added to each of thesubsamples and where the individual primer pairs are adapted to amplify, in oneamplification reaction, in each case different part-sequences of the at least onepredetermined sequence, e) carrying out an amplification reaction with each of the atleast two subsamples obtained in step d), f) determining the number of differentamplificates obtained by the amplification reactions in step e) for the individual
subsamples, and determining the number of subsamples with which in each case identicalamplificates have been obtained.
申请人：BECKMAN COULTER, INC.
地址：250 South Kraemer Boulevard Brea, CA 92821 US
国籍：US
代理机构：Müller-Boré & Partner Patentanwälte
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