DETECTION OF IMMOBILIZED NUCLEIC ACID

专利名称：DETECTION OF IMMOBILIZED NUCLEIC

ACID

发明人：Matthew Paul Beaudet,W. Gregory

Cox,Stephen Yue

申请号：US13525690申请日：20120618

公开号：US20130011927A1公开日：20130110

摘要：The present invention provides methods for determining the presence ofimmobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives ofthiazole orange, a staining solution and select fluorogenic compounds that arecharacterized as being essentially non-genotoxic. The methods comprise immobilizingnucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid orsemi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyaninedye compound and then illuminating the immobilized nucleic acid with an appropriatewavelength whereby the presence of the nucleic acid is determined. The cyanine dyecompounds are typically present in an aqueous staining solution comprising the dyecompound and a tris acetate or tris borate buffer wherein the solution facilitates thecontact of the dye compound and the immobilized nucleic acid.

申请人：Matthew Paul Beaudet,W. Gregory Cox,Stephen Yue

地址：Eugene OR US,Portland OR US,Eugene OR US

国籍：US,US,US

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