pMTV5-His B昆虫表达载体说明

编号\r  

北京华越洋生物KCVECT125\r  

pMT/V5-­‐His\r  B载体基本信息

载体名称: 质粒类型: 高拷贝/低拷贝:

启动子: 克隆方法: 载体大小: 5' 测序引物及序列: 3' 测序引物及序列:

载体标签: 载体抗性: 筛选标记: 备注: 稳定性: 组成型: 病毒/非病毒:

pMT/V5-His B 昆虫细胞表达载体 --

载体名称\r  

pMT/V5-­‐His\r  B\r  

P-MT (Metallothionein) 多克隆位点，性内切酶 3.5 kb

Metallothionein: 5'd[CATCTCAGTGCAACTAAA]3' --

V5 (Cterm), His (Cterm) Ampicillin -- -- -- 诱导型 非病毒

pMT/V5-­‐His\r  B载体质粒图谱和多克隆位点信息

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pMT/V5-­‐His\r  B载体简介\r  

The\r  DES-­‐Inducible\r  Kit\r  provides\r  the\r  expression\r  vector\r  pMT/V5-­‐His\r  and\r  a\r  choice\r  of\r  Blasticidin\r  (pCoBlast)\r  or\r  hygromycin\r  (pCoHygro)\r  selection.\r  This\r  vector\r  uses\r  the\r  Drosophila\r  metallothionein\r  gene\r  promoter\r  that\r  is\r  induced\r  in\r  S2\r  cells\r  upon\r  addition\r  of\r  copper\r  sulfate\r  or\r  cadmium\r  chloride\r  to\r  the\r  culture\r  medium.\r  \r  

The\r  pMT/V5-­‐His\r  vector\r  offers\r  the\r  following\r  features:\r  \r  

Small\r  size\r  (3.5\r  kb)\r  to\r  improve\r  DNA\r  yields\r  and\r  increase\r  subcloning\r  efficiency\r  C-­‐terminal\r  V5\r  epitope\r  tag\r  for\r  rapid\r  detection\r  with\r  Invitrogen's\r  Anti-­‐V5\r  Antibody\r  

C-­‐terminal\r  6xHis\r  tag\r  for\r  simple\r  purification\r  of\r  recombinant\r  fusion\r  proteins\r  using\r  nickel-­‐chelating\r  resin\r  

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To\r  facilitate\r  cloning,\r  a\r  set\r  of\r  three\r  vectors-­‐A,\r  B,\r  and\r  C-­‐is\r  provided.\r  Each\r  vector\r  has\r  the\r  multiple\r  cloning\r  site\r  in\r  a\r  different\r  reading\r  frame\r  relative\r  to\r  the\r  coding\r  sequence\r  of\r  the\r  C-­‐terminal\r  tag.\r  \r  

pMT/V5-­‐His\r  B载体序列\r  

TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGT AAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTA ACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAA GGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGG CCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGT TTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAGTGAATTAATTCGTTGCAGGACAGGATGTGGTG CCCGATGTGACTAGCTCTTTGCTGCAGGCCGTCCTATCCTCTGGTTCCGATAAGAGACCCAGAACTCCGGCC CCCCACCGCCCACCGCCACCCCCATACATATGTGGTACGCAAGTAAGAGTGCCTGCGCATGCCCCATGTGCC CCACCAAGAGTTTTGCATCCCATACAAGTCCCCAAAGTGGAGAACCGAACCAATTCTTCGCGGGCAGAACAA AAGCTTCTGCACACGTCTCCACTCGAATTTGGAGCCGGCCGGCGTGTGCAAAAGAGGTGAATCGAACGAAAG ACCCGTGTGTAAAGCCGCGTTTCCAAAATGTATAAAACCGAGAGCATCTGGCCAATGTGCATCAGTTGTGGT CAGCAGCAAAATCAAGTGAATCATCTCAGTGCAACTAAAGGGGGGATCTAGATCGGGGTACCTACTAGTCCA GTGTGGTGGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCGCGGTTCGAAGGT AAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAGTT TAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAAGATCCAGACATGATAAGATACATTGATGAGTTTGGAC AAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAA CCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGG TGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCAGTCGACCTGCA GGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCAC ACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTG CGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCG CGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG GATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAA TCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGA TCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTAC CATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACC AGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTT

GCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCG TGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGAT CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAG TGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTG TGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGT CAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGC GAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTT CAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAA TAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTC CCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCA CGAGGCCCTTTCGT

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pMT/V5-­‐His\r  B其他昆虫表达载体：

pMT/BioEase-DEST pXINSECT-DEST38 pIEX/Bac-1 pMT/V5-His-TOPO pVL1392 pBacPAK9 pFastBacHT B pMT/BiP/V5-His A pVL1393 pBacPAK8 pFastBacHT A pCoHygro pXINSECT-DEST39 pIB/V5-His-TOPO pFastBac1 pAc5.1/V5-His C pAc5.1-V5-His A pCoBlast pIZT/V5-His pMT/BiP/V5-His B pIEXBac-c-EGFP-1 pBlueBacHis2 C pFBDM pMT/V5-His C pAc5.1b

pFastBac-N-GST-TEV

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pAc5/V5-HisA pAc5.1/V5-His B pMIB/V5-His C pIEx/Bac-4 pIEXBac-c-EGFP-4 pBlueBacHis2 B pUCDM pMT/V5-His B pAc5.1a pFastBacI-Gus

pAc5-V5-HisB pIZ/V5-His pMIB/V5-His A pIEx/Bac-3 pAc5.1B-EGFP pFastBacHT C pFastBac Dual pMT/V5-His A

pVL1392-XyIE control vector pFastBacHT-CAT

pAc5-V5-HisC pIB/V5-His pMT/BiP/V5-His C pIEXBac-c-EGFP-3 pBlueBacHis2 A pMT-Bip-V5-HisA pMIB/V5-His B pAcGP67A pFastBac-c-His-TEV pBADZ-His6Cre