Methods and compositions for the preparation and u

专利名称：Methods and compositions for the

preparation and use of fixed-treated cell-lines and tissue in fluorescence in situhybridization

发明人：Paola Capodieci,Jon Edelson申请号：US11283062申请日：20051117

公开号：US20060141502A1公开日：20060629

摘要：This invention relates to methods for the detection of one or more mRNAtranscripts in paraffin-embedded tissue by “mRNA liberation in fixed-treated tissue or‘MLIFTT’”. This method includes treating the tissue with ammonia-ethanol andsodium borohydride combined with pressure cooking of the tissue. The chemicaltreatments reduce the tissue autofluorescence and the physical treatments overcomethe interference created by the fixation-induced chemical bonds. The methods of thepresent invention can be utilized to identify a plurality of mRNA transcripts in amicroarray format.

申请人：Paola Capodieci,Jon Edelson

地址：New York NY US,Scarsdale NY US

国籍：US,US

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