Efficient methods and apparatus for high-throughpu

专利名称：Efficient methods and apparatus for high-throughput processing of gene sequencedata

发明人：Tony Nick Frudakis申请号：US099059申请日：20010926

公开号：US20030171875A1公开日：20030911

专利附图：

摘要：One disclosed method of processing gene sequence data includes the steps ofreading gene sequence data corresponding to a gene sequence and coding sequence

data corresponding to a plurality of coding sequences within the gene sequence;identifying and storing, by following a set of primer selection rules, primer pair datawithin the gene sequence data for one of the coding sequences; repeating the acts ofidentifying and storing such that primer pair data are obtained for each sequence of theplurality of coding sequences; and simultaneously amplifying the plurality of codingsequences in gene sequences from three or more of individuals using the identified pairsof primer sequences. The set of primer selection rules include a rule specifying that all ofthe primer pair data for the plurality of coding sequences be obtained for a

predetermined annealing temperature, which allows for the subsequent simultaneousamplification of sequences from hundreds of individuals in a single amplification run.

申请人：FRUDAKIS TONY NICK

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