专利名称：QUANTITATIVE DETECTION OF NUCLEIC
ACID AMPLIFICATION PRODUCTS
发明人：BLAIR, James, Ninian, Rowell 39 Old Church
Road,DOWNES, Malcolm, John,PARRY, David,Alun Flat 2,CROSS, Lisa,ROBINSON, Philip,Steven 98 Winslow Road Wingrave
申请号：EP92911283.0申请日：19920605公开号：EP0668929A1公开日：19950830
摘要：A method of amplifying and quantitating a specific nucleic acid sequence in asample using at least one oligonucleotide primer, a supply of nucleotides and optionallya probe complementary to part of the sequence, one of these including a radionuclide. Inthe presence of a polymerase the reagents form an amplified product based on thespecific nucleic acid sequence. The amplified sequence is allowed to bind to a solidmaterial comprising a fluorescer which fluoresces in proximity to the radionuclide andfluorescence is observed as a measure of the specific nucleic acid sequence. The methodcan be applied to a range of well-known nucleic acid amplification techniques such as thePolymerase Chain Reaction. A variation on the above method allows use of the LigaseChain Reaction technique.
申请人：AMERSHAM INTERNATIONAL plc
地址：Amersham Place Little Chalfont Buckinghamshire HP7 9NA GB
国籍：GB
代理机构：Pennant, Pyers, et al
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