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ColonyDoc-It™ Imaging Station

Instruction Manual

UVP, LLC

2066 W 11th Street, Upland, CA 91786 Tel: (800) 452-6788 / (909) 946-3197 Fax: (909) 946-3597 Email: info@uvp.com

Web site: uvp.com

Ultra-Violet Products Ltd.

Unit 1, Trinity Hall Farm Estate

Nuffield Road, Cambridge CB4 1TG UK Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561

Email: uvp@uvp.co.uk

81-0294-01 Rev. D

ColonyDoc-It Imaging Station

END USER LICENSE AGREEMENT

PLEASE READ THE FOLLOWING AGREEMENT CAREFULLY

This Agreement is between UVP, LLC of 2066 West 11th Street, Upland, California 91786 (hereinafter “Licensor”) and the end user of UVP software (hereinafter “Licensee”).

Licensor has developed and offers to Licensee on a non-exclusive basis pursuant to the terms and conditions set forth

hereinafter, the following software, including related copyrighted instructional materials, (collectively referred to hereafter as “The Software”):

DOC-IT COLONY SOFTWARE

The terms of this Agreement apply without regard for the method by which the Software is acquired by Licensee. While the most common medium for acquiring the Software from Licensor is by CD-ROM, the Software may, in some instances, be acquired by download; from a Licensor thumb drive acquired from Licensor; from a CD ROM acquired from Licensor from a network location; or may be pre-installed on the Licensee‟s computer.

By using the Software, you are agreeing to be bound by all terms of this License. If you do not agree to the terms of the License, you are not authorized to use the Software in any manner.

LICENSE

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RESTRICTIONS

The Software contains copyrighted materials, trade secrets, and other proprietary material. You may not re-sell, decompile, reverse engineer, disassemble or otherwise reduce the Software to a human-perceivable form. Except as provided for in this License, you may not copy, modify, network, rent, lease, or otherwise distribute the Software; nor can you make the Software available by “bulletin boards”, on-line services, remote dial-in, or network or telecommunications links of any kind; nor can you create derivative works or any other works that are based upon or derived from the Software in whole or in part. You may not transfer the license rights to the Software to another party.

TERMINATION

This License is effective until terminated by either party. You may terminate this License at any time by returning the Software to Licensor or destroying any permanent form of the software and all related documentation and all copies and installations thereof, whether made under the terms of this License or otherwise. This License will terminate immediately without notice from Licensor if you fail to comply with any provision of this License. Upon termination, you must destroy or return to Licensor any permanent form of the software and related documentation.

LIMITED WARRANTY AND DISCLAIMER

Licensor warrants the software and related documentation to be free from defects in materials and workmanship, under

normal use for a period of ninety (90) days from the date of purchase as evidenced by a copy of the sales receipt or packing slip. Licensor‟s entire liability and licensee‟s exclusive remedy will be replacement of the defective software and related documentation or refund of the purchase price (at licensor‟s election) upon return of the software and related documentation to licensor with a copy of proof of purchase. This warranty gives you specific legal rights and you may also have other rights which vary from jurisdiction to jurisdiction. You expressly acknowledge and agree that use of the software is at your sole risk after the ninety (90) days. The software and related documentation are provided without warranties and/or conditions of any kind either express or implied, except as provided above. Licensor expressly disclaims all other warranties and/or

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regarding the use or the results of the use of the software and related documentation in terms of their correctness, accuracy, reliability, or otherwise. The limitations of liabilities described in this section also apply to the third party suppliers of

materials used in the software. No oral or written information or advice by licensor or by representatives of licensor shall

ColonyDoc-It Imaging Station

create warranties, and/or conditions, or in any way increase the scope of this limited warranty. Licensee assumes the entire cost of all necessary servicing, repair or correction after the ninety (90) days. Some jurisdictions do not allow the exclusion of implied warranties, so the above exclusion may not apply to you.

LIMITATION OF LIABILITY

Under no circumstances, including negligence, shall licensor be liable for any special or consequential damages that result from the use of, or the inability to use, the software or related documentation, even if licensor or authorized representative of licensor has been advised of the possibility of such damages. Some jurisdictions do not allow the limitation or exclusion or liability or consequential damages, so the above limitations or exclusion may not apply to you. In no event shall licensor‟s total liability to you for all damages, losses, and causes of action (whether in contract, tort (including negligence) or otherwise) exceed the amount paid by you for the software.

INTENDED USE

If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

GOVERNING LAW AND SEVERABILITY

This License shall be governed by and construed in accordance with the laws of the State of California, without giving effect to any principles of conflicts of law. Any actions, suits or proceedings instituted in connection with this License, the Software and/or related documentation shall be instituted and maintained exclusively in the Superior Court for the State of California, County of Los Angeles, or in the United States District Court for the Central District of California. By entering this License you hereby consent to the venue and jurisdiction of the aforesaid courts. If any provision of this license shall be unlawful, void, or for any reason unenforceable, then that provision shall be deemed severable from this License and shall not affect the validity and enforceability of any remaining provision. This is the entire agreement between the parties relating to the subject matter herein and shall not be modified except in writing, signed by both parties.

ColonyDoc-It Imaging Station

Table of Contents

Introduction ............................................................................................................................................................................... 7

Components ............................................................................................................................................................................. 7 System Requirements............................................................................................................................................................... 8

System Specifications............................................................................................................................................................... 8 Installing the ColonyDoc-It ........................................................................................................................................................ 9

Installing the Hardware ............................................................................................................................................... 9

Installing the Software .............................................................................................................................................. 11 Registering the Software .......................................................................................................................................... 11

Counting Colonies .................................................................................................................................................................. 13

Quick Capture........................................................................................................................................................... 13

Automatic Counting .................................................................................................................................................. 15 Manual Counting ...................................................................................................................................................... 16

Creating Templates .................................................................................................................................................. 20

User Defined Template Counting ............................................................................................................................. 21

Fluorescent Colony Counting ................................................................................................................................... 22 Zone Analysis ........................................................................................................................................................... 24

Renumbering Colony/Zone Values ........................................................................................................................... 28 Spiral Plate Counting ................................................................................................................................................ 29

Editing Colonies ...................................................................................................................................................................... 31

Add Colonies ............................................................................................................................................................ 31

Delete Colonies ........................................................................................................................................................ 32 Manual Split Colonies ............................................................................................................................................... 33

Auto Split Colonies ................................................................................................................................................... 34

Merging Colonies...................................................................................................................................................... 35

Using the Software ................................................................................................................................................................. 36

Changing Filter Settings ........................................................................................................................................... 36

Preferences .............................................................................................................................................................. 37 Toolbars ................................................................................................................................................................... 39

Reporting Functions................................................................................................................................................................ 40

Classes..................................................................................................................................................................... 40

Colonies ................................................................................................................................................................... 41 Statistics ................................................................................................................................................................... 41

Distribution................................................................................................................................................................ 42

Exporting to Excel..................................................................................................................................................... 42

Supporting 21 CFR Part-11 Compliance................................................................................................................................. 44

Purpose .................................................................................................................................................................... 44

Features ................................................................................................................................................................... 44

ColonyDoc-It Imaging Station

Usage ....................................................................................................................................................................... 44 Cleaning and Care.................................................................................................................................................... 45 Replacing Switches .................................................................................................................................................. 46

Servicing the ColonyDoc-It ..................................................................................................................................................... 45

Replacing Fuses ....................................................................................................................................................... 46 Ordering Replacement Parts and Accessories ......................................................................................................... 46

Troubleshooting ........................................................................................................................................................ 46 Return Service Procedure ........................................................................................................................................ 47

Technical Support..................................................................................................................................................... 48

Warranty ................................................................................................................................................................................. 48

ColonyDoc-It Imaging Station

Introduction

This manual covers the installation and user instructions for the ColonyDoc-It Imaging Station.

The innovative, compact design enables users to process automated, fast and accurate colony counting. The high resolution digital color camera allows users to capture white light and fluorescent marked colonies and a wide array of samples. The sophisticated yet intuitive system offers researchers easy detection and analysis of media.

The software loads on the user‟s computer for camera control, image capture and colony counting. The software provides automatic and manual counting capabilities plus user defined templates for speeding up research

experiments. Users can define specific counting parameters including color differentiation and filter identification by group or size. Once the colonies are counted, the results display on the screen. Images and data can be saved in multiple formats and the data can be exported to Excel. The software supports 21 CFR Part 11 compliance.

Components



Colony counter Black plate USB cord Power cord Software CD



ColonyDoc-It Imaging Station

System Requirements



Operating System: Windows XP Professional with Service Pack-2 (32 bit only), Vista (32 bit only), Windows 7 (32 bit only)

Internet Explorer 6.0 or higher (To determine the version of Internet Explorer, open Internet Explorer and click on Help > About)

Minimum resolution: 1024 x 768

Intel Pentium Processor or equivalent, 800 mHz or higher 512 MB of RAM or greater

200 MB of available hard disk space for the program, more for data

To avail the functionality of 21 CFR Part 11, then the partition must be formatted with NTFS. CD-ROM drive



 



System Specifications

Electrical ratings:

Model Numbers:

97-0539-01, 97-0539-04 100-115V, 60Hz, 0.25 Amps 97-0539-02, 97-0539-05 230V, 50Hz, 0.13 Amps

Model Numbers:

Operational Ratings:

Unit intended to be used indoors Altitude must not exceed 2000 m

Operating temperatures not to exceed 0°C to 40°C (32°F to 104°F) Operating humidity not to exceed 10-90%

ColonyDoc-It Imaging Station

安装 ColonyDoc-It

Installing the Hardware 硬件安装

将ColonyDoc-It Imaging Station放置在水平表面上。

将电源线的一端插入ColonyDoc-It Imaging Station背面，另一端插入电源插座。为安全起见，请确保所选位置的电源能够及时切断。

将机器背面I/0开关切换至I侧，启动ColonyDoc -It。

将USB数据线的两段分别接入ColonyDoc-It和

电脑USB端口。

卸除门扇

ColonyDoc-It的门扇可通过手动松动各门内侧的两个螺丝来卸除。

ColonyDoc-It Imaging Station

安装过滤器

注：过滤器为可选附件，这一步仅适用于需要过滤器的系统。过滤器托盘安装在机体顶部的滤光盒中。拿出过滤器托盘：旋出滤光盒上的四个黄铜螺丝，拉出滤光盒。

旋出将滤光框固定在滤光盒上的两个螺丝。

把过滤器放在开口上方。

装回滤光框和螺丝。

重新安装滤光盒。

注：当机体装有滤光盒时，可以安装过滤器。请勿触碰过滤器的亮面。

ColonyDoc-It Imaging Station

软件安装

将Doc-It菌落计数器光盘（Doc-It Colony Counter CD）插入电脑光驱。

弹出安装向导（InstallShield Wizard）窗口。

按照安装说明进行安装。

安装完成后，请参考本指南中的软件注册（Registering the Software），激活软件。

软件注册

打开软件程序

双击电脑桌面上的软件图标。

需要注册后方能激活软件。选择立即激活（On the fly activation），通过互联网立即激活软件。如电脑没有接入互联网，请参照离线激活（Offline activation）说明，或致电UVP注册软件。

单击下一步（Next）继续。

ColonyDoc-It Imaging Station

填写所有表格所需信息。

填写CD光盘上的序列号（Serial Number）。序列号由四组六位数字组成。单击获取激活号码（Get Activation No.），激活号码（Activation

Number）出现在文字框中时单击激活（Activate）。

已获得激活号码（Already have an

activation ID）用于获取初始激活码后软件的重装。

如果电脑没有接入互联网，单击离线激活（Offline activation）注册软件。用户可在下次使用时输入获取的激活号码。

单击下一步（Next）继续。

单击显示的链接并填表，之后会显示说明。单击完成（Finish）。

ColonyDoc-It Imaging Station

菌落计数

快速拍照

把培养皿放在透照板上。

选择恰当的光照选项。如果不确定，将（位于菌落计数器前侧的）选择开关调至底座照明 >透射（Base Lighting > Trans）并移开黑色平板（如有）。透射照明适用于大多数情况。

如果菌落计数器没有打开，双击电脑桌面上的Doc-It 菌落计数器图标（Doc-It Colony Counter icon）。

相机控制（Camera Control）模块处于激活状态，如下图绿色圆圈表示的电脑截图所示。如未激活相机控制确保

模块（灰色字体标示），请参考故障排除章节：非活动相机窗口（Inactive Camera Window）。

在相机控制（Camera Control）模块中设定培养皿直径，有6cm，10cm和15cm三种选择。

选择培养皿最小菌落（smallest colony）尺寸，有<1mm和1mm+两种选项。如最小菌落尺寸小于1mm，选择<1mm。

在菌落照明（Colony Lighting）项下选择白光（White Light）。从暗淡（Dim）到明亮（Bright）的滑块仅在选中荧光菌落照明（Fluorescent Colony Lighting）选项时方能激活。如果要对荧光菌落计数，参考本指南的荧光菌落计数（Fluorescent Colony Counting）章节。

ColonyDoc-It Imaging Station

把培养皿插入菌落计数器中。

单击相机控制（Camera Control）模块中的预览（Preview）按钮，移动培养皿使其位于红圈中心中心。外侧红圈应恰好位于培养皿外边缘的内侧。如不可行，请核对培养皿直径（Plate Diameter）是否选择正确。

单击相机控制（Camera Control）模块中的拍摄（Capture）按钮进行拍摄。单击屏幕左侧菌落计数（Colony Count）模块中的开始菌落计数（Start Colony Count），开始计数。

选择自动计数或者手动计数（Automated Count or Manual Count）。要完成自动计数d的设定，参考本指南自动计数（Automatic Count）章节。要完成手动计数的设定，参考本指南的手动计数（Manual Count）章节。

注：由于未创建模板，用户自定义模板计数（User Defined Template Count）最初显示为灰色。模板计数功能可让用户针对频繁计数的培养皿创建计数模板。进行手动计数后方可对模板进行设定。

技巧：如不进行荧光菌落计数，ColonyDoc-It的门扇可轻松移除。参考移除ColonyDoc-It门扇（Removing ColonyDoc-It Doors）章节。

ColonyDoc-It Imaging Station

照明技巧：

培养皿的照明有四个选项。有白光（White）或蓝光（Blue）的头顶照明（Epi Lighting），光线从培养皿顶部照射。有透射（Trans）或暗视野（Darkfield）的底座照明（Base Lighting），光线从培养皿底部照射。蓝光用于激活绿荧光蛋白染色的菌群。

大多数通过涂布培养法，使用透明基质（细菌酵母或霉菌）的样本最适用底座照明 >透射（Base Lighting > Trans）。

如培养皿底部有很多笔迹，有薄膜纸或贴纸，可使用头顶照射>白光（Epi Lighting > White）或底座照射>暗视野（Base Lighting > Darkfield）。

计数倾注平皿时，使用底座照射>暗视野（Base Lighting > Darkfield）。

把黑色平板盖在透照板上并使用头顶照射（EpiLighting）可获得更好的对比度，从而提高了计数准确度。

制作样本：

制作样本时，避开中心，在培养皿底部边缘标记，以增加计数精确度。

自动计数

在进行自动计数前，确保已捕捉培养皿图像，且该图像符合分析标准。参考本指南的快速拍照（Quick Capture）说明。

单击屏幕左侧的开始菌落计数（Start Colony Count），将弹出新的选择菌落计数类型（Colony Count Type Selection）窗口。选择自动计数（Automated Count）并单击确定（OK）。

ColonyDoc-It Imaging Station

自动计数结果显示在屏幕左侧。

菌落可以红色（如图）或轮廓线显示。菌落还能以多种颜色编号或注释。参考本指南的首选项（Preferences）章节获取更多关于更改图像显示详情。

可根据情况增加、删除、分离或合并菌落。参考本指南相关章节。

手动计数

在进行手动计数前，确保已捕捉培养皿图像，且该图像符合分析标准。参考本指南的快速拍照（Quick Capture）说明。

单击屏幕左侧的开始菌落计数

（Start Colony Count），将弹出新的选择菌落计数类型（Colony Count Type Selection）窗口。选择手动计数（Manual Count）并单击确定（OK）。弹出手动计数向导（Manual Count Colony Wizard）窗口。

ColonyDoc-It Imaging Station

第一步：选择种类

选择手动计数（Manual Count）后将出现手动计数向导（Manual Count Colony Wizard）窗口，包含两个标签：

第一个标签一是“两步中的第一步：选择

种类”（Step 1 of 2: Select Classes），第二个标签是“两步中的第二步：完成”（Step 2 of 2: Finish）。

第一个标签用于界定培养皿内部需要分析的感兴趣区。感兴趣区用绿色圆圈标示，应覆盖全部有用的菌落。软件会自动界定感兴趣区，但用户可通过单击界定计数区域

（Define Counting Region），根据需要增加或减小圆圈尺寸。

如要更改计数区域的大小，单击界定计数区域（Define Counting Region），拖动任一角落指针改变圆圈大小。

ColonyDoc-It Imaging Station

在选择种类标签里，用户可以选择培养皿里种类和菌落的数量。

计算所需菌落应首先确保增加点（Add Points）按钮已标为黄色高亮，单击选择一个菌落（click

on a colony），在培养皿原始图像（original image）中进行计数。（在“两步中的第一步：选择种类”标签中有原始图像的复制小图，但无法进行菌落选择）。

种类（Classes）被定义为样本里出现的各种类型的细菌、酵母和霉菌。该软件可以在同一个培养皿里检测出多个种类。若需增加种类，单击窗口中种类（Classes）栏里的添加（Add）按钮。

Click on a colony

一旦选择了第一个菌落，“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）窗口即显示一张黑色背景的图片，包含与分析细节（Analysis Details）窗口中所选点颜色相同的所有菌落。

要从分析细节（Analysis Details）窗口中删除点或者种类，高亮显示该点，选择删除点（Remove

Point）或者高亮显示该种类，并选择删除（Remove）。

如需要，继续单击菌落以增加点，直到黑白图像以白色显示全部有用菌落。

单击计数（Count）按钮进入下一个标签。

注：“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）标签中的设置可存为自定义模板。如未创建模板，下拉菜单中仅会显示缺省（Default）一个选项。创建模板参考本指南中的模板（Templates）章节。

ColonyDoc-It Imaging Station

第二步：完成

单击计数（Count）按钮后，软件会自动进入下一个标签“两步中的第二步：完成”（Step 2 of 2: Finish）。

如需筛选菌落，在筛选菌落（Filter colonies）栏中调整菌落的形状及大小。

拖动滑块按钮改变形状（shape）【从圆形（circular）到不规则图形（irrgular）】和大小（size）【1像素（1 pixel）到200像素（200 pixels）】，以捕捉额外菌落或剔除菌落。

该窗口为将设置存入模板的第二种方法。创建模板参考本指南中的模板（Templates）章节。单击完成（Finish）退出手动计数模式，获取最终计数结果。

ColonyDoc-It Imaging Station

创建模板

用户可在模板中设定计数参数，以便快速连续地计数属性相似的培养皿。

要为任何培养皿创建模板，在菌落计数（Colony Count）模块中单击开始菌落计数（Start Colony Count）【如培养皿曾被计数，单击重新开始菌落计数（Restart Colony Count）】并选择手动计数（Manual Count）功能。在“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）或“两步中的第二步：完成（Step 2 of 2: Finish）”的操作中都可以存储模板。

如在“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）中存储模板，添加所需点和种类（如未选中），并单击窗口中模板（Template）栏里的新建（New）按钮。

更多信息参考本指南的手动计数（Manual Count）说明。

新模板（New Template）窗口会弹出，输入新的模板名（Template name）。

输入新名字，单击确定（OK）。

模板名窗口将自动关闭并跳转回“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）标签。

单击计数（Count）按钮进入“两步中的第二步：完成”（Step 2 of 2: Finish）标签。

ColonyDoc-It Imaging Station

如为捕获更多菌落而移动了形状（shape）和大小（size）滑块，单击窗口中模板（Templates）栏里的创建模板（Create template），保存新设置。

选择完成（Finish）结束计数并保存模板。

注：可直接在“两步中的第二步：完成”

（Step 2 of 2: Finish）中保存模板。用户可跳过“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）中的模板创建选项而直接进入第二步。

注：如有以前保存的模板，软件将在手动计数过程中自动使用该模板。

用户自定义模板计数

在进行下个步骤前，应确保在手动计数过程中已保存模板。如无模板，参考本指南创建模板（Creating Templates）章节进行模板创建。

选择开始菌落计数（Start Colony Count），然后选择用户自定义模板计数（User Defined Template Counting），从下拉菜单中选择所需模板。单击确定（OK）。

ColonyDoc-It Imaging Station

新窗口将显示菌落计数总数（Total colonies）。

荧光菌落计数

确保门扇附于机体或关闭周围的灯光。打开头顶照明（Epi Lighting）开关，选择蓝色（Blue）。

将绿荧光蛋白发射过滤器【Green Fluorescent Protein (GFP) emission filter】插入任一托盘（参考本指南安装过滤器（Installing the Filter）章节说明），确保过滤器置于相机下方。（如果过滤器位于该位置，移动滑块至正确位置。）

将绿荧光蛋白染色的样品插入ColonyDoc-It。

ColonyDoc-It Imaging Station

在Doc-It Colony Counter软件中选择合适的平板直径（Plate Diameter）、最小菌落（Smallest Colony的荧光（）Fluorescent信息以及菌落照明（）照明功能。Colony Lighting ）可在暗淡（Dim）和明亮（Bright）间调整滑块已达到最佳效果。

选择预览（Preview），将红圈辅助线内的平板调至合适的位置，然后选择拍照（Capture）。

最后选择自动、手动或使用模板进行菌落计数。详情参考本指南自动计数、手动计数或模板计数（CountAutomatic Count, Manual Count, ）章节。

or Template 23

ColonyDoc-It Imaging Station 区域分析

区域分析可为用户提供区域大小信息，信息可应用于抑菌圈分析等。

区域分析过程开始前，确保已捕捉培养皿图像，且该图像符合分析标准。图像捕捉详情参考本指南快速拍照（Quick Capture）章节。

为获取平板区域分析信息，单击复选框中的区域（Zone），然后选择开始菌落计数（Start Colony Count）。

选择手动（Manual）或使用用户自定义（User Defined）方法进行计数。单击确定（OK）。

如首次使用该软件，则无已创建模板，用户自定义模板计数（User Defined

Template Count）选项呈灰色。想要创建模板需首先运行手动计数（Manual Count）功能以保存设置。

First click on Zone Second click on Start Colony Count

ColonyDoc-It Imaging Station

第一步：选择种类

选择手动计数（Manual Count）后将出现手动菌落计数向导（Manual Count

Colony Wizard）窗口，包含两个标签：第一个标签是“两步中的第一步：选择种类”（Step 1 of 2: Select Classes），第二个标签是“两步中的第二步：完成” （Step 2 of 2: Finish）。

第一个标签用于界定培养皿内部需要分析的感兴趣区。感兴趣区用绿色圆圈标示，应覆盖全部有用的区域。软件会自动界定感兴趣区，但用户可通过单击界定计数区域

（Define Counting Region），根据需要增加或减小圆圈尺寸。

如要更改计数区域的大小，单击界定计数区域（Define Counting Region），拖动任一角落指针改变圆圈大小。

ColonyDoc-It Imaging Station

在选择种类标签里，用户可以选择培养皿里种类和区域的数量。

分析所需区域应首先确保增加点（Add Points）按钮已标为黄色高亮，单击选择一个区域（click on a zone），在培养皿原始图像（original image）中进行计数。（在“两步中的第一步：选择种类”标签中有原始图像的复制小图，但无法进行区域选择）。

Click on a zone

一旦选择了第一个区域，“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）窗口即显示一张黑色背景的图片，包含与分析细节（Analysis Details）窗口中所选点颜色相同的所有区域。

如需要，继续单击区域以增加点，直到黑白图像以白色显示全部感兴趣区。

要从分析细节（Analysis Details）窗口中删除点或者种类，高亮显示该点，选择删除点（Remove

Point）或者高亮显示该种类，并选择删除（Remove）。

“两步中的第一步：选择种类”（Step 1 of 2: Select Classes）标签中的设置可存为自定义模板。如未创建模板，下拉菜单中仅会显示缺省（Default ）一个选项。创建模板参考本指南中的模板（Templates）章节。

单击计数（Count）按钮进入下一个标签。

ColonyDoc-It Imaging Station

第二步：完成

单击计数（Count）按钮后，软件会自动进入下一个标签“两步中的第二步：完成”（Step 2 of 2: Finish）。

增减区域可拖动滑块按钮改变形状（shape）【从圆形（circular）到不规则图形（irrgular）】和大小（size）【1像素（1 pixel）到200像素（200 pixels）】，直至高亮显示全部感兴趣区。

该窗口为将设置存入模板的第二种方法。创建模板参考本指南中的模板（Templates）章节。单击完成（Finish）退出手动计数模式，观察结果表。

软件将返回初始界面并显示界定区域数目。

观察区域分析数据，点击菌落计数（Colony Count）模块中主工具栏（Main Tools）部分的显示结果窗口（Show Results Window）。随后将出现提供种类、区域（被列为菌落）、统计数据、区域分布相关信息的窗口。在报表中，用户可观察区域面积和周长。

报表功能详见本指南报表功能（Reporting Functions）章节。

ColonyDoc-It Imaging Station

重新编号菌落/区域值

为简化统计报表，可对各菌落或区域的赋值进行重新编号。

单击栅格分析工具栏（Grid Analysis Tools）下的重新计算菌落标签

（Recompute Colony Labels），对各菌落或区域的赋值进行重新编号。注：应先对平板进行计数。

重新计算菌落标签（Recompute Colony Labels）功能将对平板从左往右、从上往下进行读数。因此标示为1的菌落（或区域）将位于左侧并朝向平板顶部。

运用重新计算菌落标签（Recompute Colony Labels）功能前

运用重新计算菌落标签（Recompute Colony Labels）功能后

ColonyDoc-It Imaging Station

螺旋板计数

为运行螺旋板计数，单击复选框中的螺旋（Spiral），然后选择开始菌落计数（Start Colony Count）。

接下来选择自动计数（Automated Count）、手动计数（Manual Count）或用户自定义模板计数（User Defined Template Count）。

First click onto Spiral

Second click onto Start Colony Count

注：如首次使用该软件，则无已创建模板，用户自定义模板计数（User Defined Template

Count）选项呈灰色。想要创建模板需首先运行手动计数（Manual Count）功能以保存设置。

详见本指南自动计数、手动计数或模板计数（Automated Count, Manual Count, or Template Count）运行说明。

进行计数后将出现螺旋板分析（Spiral Plate Analysis）窗口，其中计数图像上方会出现绿色网格。

如要更改覆盖网格的尺寸，可将覆盖层尺寸（Overlay Size）滑块移至左侧（减小网格尺寸）或右侧（增加网格尺寸）。如要计算SPLC/mL，输入或使用总存放体积（Total volume deposited）框中的向上或向下箭头。计算结果即将出现在计算SPLC/mL（Calculate SPLC/mL）按钮的下方。

ColonyDoc-It Imaging Station

如需观察螺旋板计数数据表

（Data Table）分析，单击“+”符号（随即显示扩展报表）。所列值将提供计数板各象限和部分的菌落数目。

如需保存螺旋板设置模板，单击另存为（Save As），并命名该模板。

如需打印或将数据导出至 Excel，单击打印（Print）或导出至Excel（Export to Excel）按钮。

在螺旋板分析（Spiral Plate Analysis）窗口中，用户可选择使用排列螺旋板覆盖层（Align Spiral Plate Overlay），移动绿色覆盖网格。

如需移动网格，单击排列螺旋板覆盖层（Align Spiral Plate Overlay），单击并拖动绿色网格，使其中心与所捕捉图像的中心吻合。

如需清除螺旋板分析，单击清除螺旋板分析（Clear Spiral Plate Analysis）。此举将清除以该板为中心的数据以及螺旋板网格。

可在任一计数板上进行螺旋板分析。如需对之前任一计数板进行螺旋反分析，选择排列螺旋板覆盖层（Align Spiral Plate Overlay）上方的螺旋板分析（Spiral Plate Analysis）。

ColonyDoc-It Imaging Station

Editing Colonies

增加菌落

用户可通过运行初始计数【自动计数（Automated Count）、手动计数（Manual Count）或用户自定义模板计数（User Defined Template Count）】手动增加菌落。 ), and adding colonies manually. 如需增加菌落，确保菌落计数（Colony Count）模块中的增加菌落（Add Colonies）标签呈黄色高亮显示。

如难以看见菌落，可放大该菌落。在缩放/平移

（Zoom/Pan）模块中，朝右侧“+”方向移动滑块按钮放大图像，随即在该模块中出现一个矩形物，将其移动至感兴趣区上方。矩形物界定区域内的中心培养皿图像将放大显示。

单击需要增加的菌落。在所选菌落点的周围将由出现一个圆圈（内部或外部）。

总菌落（Total colonies）计数将包括增加的菌落。

Added Colony ColonyDoc-It Imaging Station

如需使用更大的圆圈高亮显示菌落，可进行分析显示设置（Analysis Display

Settings）。一个新窗口将开启。浏览选择主设定>分析>菌落计数（Main Settings > Analysis > Colony Count）。

在窗口增加工具（Add Tool）部分，在下拉菜单中选择圆圈半径（Circle Radius ），分别为5、10、15或20。

删除菌落

用户可通过运行初始计数【自动计数（Automated Count）、手动计数（Manual Count）或用户自定义模板计数（User Defined Template Count）】手动删除菌落。

如需从总菌落（Total Colonies）计数中删除菌落，确保增加菌落（Add Colonies）标签呈黄色高亮显示。

如难以看见菌落，可放大该菌落。在缩放/平移（Zoom/Pan）模块中，朝右侧“+”方向移动滑块按钮放大图像，随即在该模块中出现一个矩形物，将其移动至感兴趣区上方。矩形物界定区域内的中心培养皿图像将放大显示。

单击需要删除的菌落。

ColonyDoc-It Imaging Station

菌落圆圈（内部或外部）将消失。总菌落（Total colonies）计数将更改至删除后的菌落数。

Deleted Colony 手动分离菌落

用户可通过运行初始计数【自动计数（Automated Count）、手动计数（Manual Count）或用户自定义模板计数（User Defined Template Count）】手动分离菌落。在两个或多个菌落十分贴近且被软件视为一个菌落的情况下，应对菌落进行分离。

如需分离菌落，确保手动分离菌落（Manual Split Colonies）标签呈黄色高亮显示。之后会出现新窗口，要求用户“在需要分离的菌落间画线，然后松开。”

如难以看见菌落，可放大该菌落。在缩放/平移

ColonyDoc-It Imaging Station

在两个或多个贴近的菌落或视为一个的菌落间画线。在按住鼠标左键的同时，利用指针从菌落一边移至另一边。

总菌落（Total colonies ）计数将包括由分离所识别的新菌落。

Split Colonies 自动分离菌落

用户可通过运行初始计数【自动计数（Automated Count）、手动计数（Manual Count）或用户自定义模板计数（User Defined Template Count）】单击菌落进行分离。在两个或多个菌落十分贴近且被软件视为一个菌落的情况下，应对菌落进行分离。

如需分离菌落，确保自动分离菌落（Auto Split Colonies）标签呈黄色高亮显示。

如难以看见菌落，可放大该菌落。在缩放/平移（Zoom/Pan）模块中，朝右侧“+”方向移动滑块按钮放大图像，随即在该模块中出现一个矩形物，将其移动至感兴趣区上方。矩形物界定区域内的中心培养皿图像将放大显示。单击需要分离的菌落。

如难以看见菌落，可放大这些菌落。参考本指南缩放/平移（Zoom/Pan）章节。总菌落（Total colonies）计数将包括由分离所识别的新菌落。

ColonyDoc-It Imaging Station

合并菌落

用户可通过运行初始计数【自动计数（Automated Count）、手动计数（Manual Count）或用户自定义模板计数（User Defined Template Count）】手动合并菌落。在一个菌落被软件视为两个单独菌落的情况下，应对菌落进行合并。只有在菌落间距小于4像素时方能进行合并。如需合并菌落，确保合并菌落（Merge Colonies）标签呈黄色高亮显示。之后会出现新窗口，要求用户“选择所需菌落，按下合并（Merge）按钮进行合并。”

如难以看见菌落，可放大该菌落。在缩放/平移

单击需要合并的菌落。

总菌落（Total colonies）计数将反映合并菌落后的变化。

Merged Colony ColonyDoc-It Imaging Station

使用软件

更改过滤器设置

为缩短用户初始设置时间，生成的过滤器仅对“真实的”菌落计数，并去除了样板中（在无过滤器的情况下）可能被计入总菌落计数的固有瑕疵。通过一些临界参数设置，过滤器可防止对某一菌落进行计数。

如需设置新过滤器参数，单击显示结果窗口>过滤器>过滤器种类（Show Results Window > Filter > Filter class）并拖动指针更改种类。

随即出现一个新的过滤菌落（Filter Colonies）窗口，显示如下参数：面积（Area）、周长（Perimeter）、平均直径（Avg Diameter）和圆度（Circularity）。

预先定义的区间已确定。可更改各参数，使其符合各类样本。

输入保留或排除的值。单击确定（OK）。软件将根据新过滤值对菌落进行重新计数。

新区间可以是包容或排斥的。根据样本需要单击保留区间（Keep Range）或排除区间（Exclude range）。

ColonyDoc-It Imaging Station

首选项

从文件> 首选项（File > Preferences）中打开首选项（Preferences）窗口。该窗口允许用户设置新的默认值，包括显示选项、标签颜色、注释、相机模板设置和其他辅助功能。

如需改变标签类型（Label Type），在分析>菌落计数（Analysis >

Colony Count）首选项窗口中单击向下箭头，选择：

 无（None）

 种类（Class）

 编号（普通）Number (general)



编号（按种类）Number (in class)

如需改变菌落标志（Colony

Marking

），单击向下箭头，选择：

 无（None）



描画轮廓（Outline）



填充（Fill）

如需改变标签颜色（Label Color），单击自动（Auto）或向下箭头选择所列颜色。

ColonyDoc-It Imaging Station

如需改变圆圈半径（Circle Radius），38

单击向下箭头，选择相应数目，更改圆圈像素范围。

相机>佳能相机（Cameras > Canon Camera开后保存修改模板设置。选择：）首选项窗口允许客户在断

 询问（Ask）



总是（Always）



从不（Never）

如需改变文本注释行为（Text Annotation Behavior

），选择：  与图像变焦同步，即图像缩小或

放大时注释也随之缩小或放大



不与图像变焦同步，即图像缩小或放大时注释保持不变

ColonyDoc-It Imaging Station

记录（Logging）首选项窗口允许用户定义日志文件路径和日志级别。如需更改日志文件路径（Log File Path），单击……并查找新目录。如需更改日志级别（Log Level），单击向下箭头，从列表中选择。

，

工具栏

工具栏允许用户只需单击鼠标即可选择大多数指令。定制的工具栏允许用户灵活地添加常用的指令，删除不常用的指令。

初始默认按钮包括：

              

打开（Open）：打开之前保存的图像文件保存（Save）：保存当前图像

另存为（Save As）：将当前图像以其他名字保存关闭（Close）：关闭软件程序打印（Print）：打印当前图像

复制（Copy）：复制所选文本、所选当前部分图像或全部图像至剪贴板粘贴（Paste）：将剪贴板项目粘贴至屏幕上

选择性粘贴（Paste Special）：将当前项目覆盖层粘贴至剪贴板剪切（Cut）：剪切特定区域

编辑注释（Edit Annotation）：选择编辑某一注释文本注释（Text Annotation）：创建文本注释直线注释（Line Annotation）：创建直线注释矩形注释（Rectangle Annotation）：创建矩形注释椭圆形注释（Ellipse Annotation）：创建椭圆注释高亮注释（Highlighter Annotation）：创建高亮注释

确定图像比例尺（Define Image Scale）：按尺寸而非像素校准图像



ColonyDoc-It Imaging Station



测量长度（Measure Length）：允许用户绘制线条并测量图像上任意两点间的距离，单位取决于空间校准。测量角度（Measure Angle）：允许用户绘制角度并测量角度值。测量面积（Measure Area）：允许用户绘制区域并测量区域面积。新感兴趣区（New ROI）：删除感兴趣活动区，新建一个该类型的区域

矩形感兴趣区（Rectangular ROI）：更改当前鼠标工具，选择矩形感兴趣区（Rectangular Region of Interest）。如当前图像已包含该区域，选定一个区域。

椭圆形感兴趣区（Elliptical ROI）：更改当前鼠标工具，选择椭圆形感兴趣区（Elliptical Region of Interest）。如当前图像已包含该区域，选定一个区域。

多边形感兴趣区（Polygonal ROI）：更改当前鼠标工具，选择多边形感兴趣区（Polygonal Region of Interest）。如当前图像已包含该区域，选定一个区域。

自由形感兴趣区（Freeform ROI）：更改当前鼠标工具，选择自由形感兴趣区（Freeform Region of Interest）。如当前图像已包含该区域，选定一个区域。

魔棒感兴趣区（Magic Wand ROI）：让用户选择一个颜色相同的区域（如一朵红花），而无需勾勒其轮廓。



报表功能

菌落计数结果可现实在结果窗口中。如需显示结果：

  

单击显示结果窗口（Show Results Window），获得菌落计数结果。培养皿菌落计数结果由屏幕左上角标签进行显示。标签包括种类、菌落、统计数据和分布。

种类

种类（Classes）标签显示了培养皿所确认的各类临界参数的相关信息。下列为种类（Classes）所报告的类别及关联值。

注：除已对平板进行校准外，所有尺寸信息均以像素为单位。



样本中的种类编号（Class Number）该种类的菌落数目

样本中该菌落分类的百分比

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ColonyDoc-It Imaging Station

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样品中该种类菌落的总面积（以像素为单位）样品中该种类菌落面积的百分比该种类菌落的平均面积（以像素为单位）面积的标准偏差

最小面积（以像素为单位）最大面积（以像素为单位）



菌落

菌落（Colonies）标签显示了培养皿所计数的各菌落的临界参数相关信息。下列为菌落（Colonies）所报告的类别及关联值。

注：除已对平板进行校准外，所有尺寸信息均以像素为单位。



种类编号

该菌落的总面积（以像素为单位）该菌落的周长（以像素为单位）该菌落的平均直径（以像素为单位）该菌落的圆度（用数字表示菌落的圆度）



统计数据

统计数据（Statistics）标签显示了统计特性和与该特性相关的面积（以像素为单位）信息。

注：除已对平板进行校准外，所有尺寸信息均以像素为单位。

ColonyDoc-It Imaging Station

在第二个窗口中，特性（Property）位列面积（Area）旁边，并列出了一些数值，包括：



最小面积（以像素为单位）最小面积菌落

最大面积（以像素为单位）最大面积菌落

面积值区间（以像素为单位）平均面积值（以像素为单位）值标准偏差

总值（以像素为单位）菌落数目

 

  

 



分布

分布（Distribution）标签用图表显示了菌落区域信息。

下拉菜单允许用户报告培养皿计数菌落的平均直径、面积、周长和圆度图形信息。用户还可更改图像中显示的容积号（Number of Bins）。（此处显示的容积号为50）

导出至Excel

如需导出数据，从菌落计数结果（Colony count results）窗口中点击文件>将结果发送至Excel（File > Send results to Excel）。以Excel格式保存文件，然后打开文件。

第一个标签显示种类（Classes）。

ColonyDoc-It Imaging Station

第二个标签显示菌落（Colonies）。

第三个标签显示统计数据（Statistics）。

ColonyDoc-It Imaging Station

Supporting 21 CFR Part-11 Compliance

Purpose

US - Food and Drug Administration (US-FDA) created and released Part 11 of Title 21 of Code of Federal Regulations (CFR) in August 1997.

The rules delineate the conditions under which the US-FDA considers electronic records and electronic signatures equivalent to paper records and paper signatures. The instructions for compliance really span the entire

organization and its practices. LS software by UVP is one piece that rightly fits into the bigger picture and supports compliance.

Note:

While software from UVP, LLC is an essential tool for assisting an organization to maintain CFR

compliance, UVP cannot claim that this is the only tool needed to achieve overall CFR compliance. The organization must establish policies and procedures that work in conjunction with such efficient tools, to ensure total compliance with 21 CFR Part 11 regulations.

Features

UVP provides software support for the following two sections of CFR regulations:

  

Section 11.10 (e) – For electronic records, this section requires the use of computer- generated, time- stamped audit-trails to track changes.

The software keeps track of all changes that affect image-data. Any action in the software that modifies the original data of an image open in the LS workspace, is logged. The log of such changes is individually maintained for each image and is referred to as „History‟ in the software.

Section 11.3 (b) (4) – This section mandates that the system be controlled by users responsible overall for contents of electronic records required to track.

The software provides an elaborate system of maintaining secure user accounts. Assign unique usernames and passwords to all the users who will be using the software. Each account can also be configured to provide read or modify access to other users‟ data. Events generated in the audit trail (above) are logged with the username.

Usage

View an Audit Trail (History)



Open the image in question. Right click on the image and select Image Information. Open the History tab.

Events are listed in the left column. Click on each event to view the entry details on the right.

Add notes to each event if required.



ColonyDoc-It Imaging Station

Print an Audit Trail (Image History)



Open the image for which an Audit Trail print copy is needed.

Go to Tools > Reports. (This option is disabled, if no printer is available.) A window opens with various types of reports available.

To select to print an Audit Trail, click the Image History item. If other reports are required, click in the checkbox of the other reports needed. For example, if both an audit trail and the image report need to be printed, click on Image Report and Image History.

Adjust the header and footer settings or printer settings if necessary, and print the trail.



Servicing the ColonyDoc-It

Cleaning and Care

Use 70% Isopropanol Alcohol to clean the ColonyDoc-It external surfaces, base, and doors.

Clean the ColonyDoc-It twice a day, before and after every use.

Cleaning the Diopter

Weekly clean the diopter with a soft photographic lens cleaning cloth dampened with lens cleaning solution. CAUTION: Do not pour the solution directly onto the diopter. The diopter is located in the filter box. To access the diopter: Remove the four brass thumbnuts on the filter box and remove the filter box from the housing.

Clean both sides of the diopter. Remove the filter if necessary to access the diopter (refer to Installing the Filter for instructions on removing the filter.)

ColonyDoc-It Imaging Station Replacing Switches

Follow these steps to replace the light switches on the cabinet. Refer to replacement parts for ordering information.

NOTE: This only applies to the light switches on the front of the system. Removal of other switches voids the manufacturer‟s warranty.

  

Unplug the cabinet from the power source prior to removing the power switch.

To remove the switch from the unit, use a thin flathead screwdriver to pry the switch out from the housing. Be careful not to scratch the paint of the unit in the process.

Once the switch is removed, a number of connector wires will be observed plugged onto the back of the unit. Normally there are two black and two white wires, with some exceptions. Pull one wire out of the old switch and put it into the same connector location on the new switch. Continue this process for each wire until all are connect to the new switch. Push the new switch into position.



Replacing Fuses

Fuses are located in the back of the unit.

   

Remove the fuse by using a flathead screwdriver to turn the fuse counter clockwise. Pull the fuse out. Insert the new fuse.

Use a screwdriver to turn the fuse clockwise to lock.

Ordering Replacement Parts and Accessories

Contact UVP or authorized distributor for replacement parts. For replacement parts or components not shown here, please call UVP Customer Service or place of purchase.

Replacement Parts

Part Number 53-0135-01

Qty Required

1 2

Order as required 1

Switch, light

Fuse, 3.15AMP/250V 5x20m, SLO BLO 56-0022-04 Thumbnut GFP Filter

62-0117-01 38-0340-01

Troubleshooting

Camera not active

Once the software loads, the Camera control window should become active. (The user should be able to select plate diameter sizes, colony sizes, and use all the functions within the Camera control window.)

If the Camera control is not active, turn the

power switch off and then back on in the back of the unit. If a window appears such as the one to the right, select Cancel.

ColonyDoc-It Imaging Station

If the window is still inactive, remove the USB cable from the USB port on the computer and then plug the USB cable back into the USB port on the computer.

If the window is still inactive, contact Technical Support.

Count not accurate

The user may add, delete, merge, and split colonies for a more accurate count.

The user may increase or decrease the count area by using the ROI tool to change the area of interest using the Automated or Manual Count.

The user may perform a manual count. Refer to the Manual Colony Counting procedures in this manual.

The user may change the pre-defined filter settings. These settings are area of the colony, perimeter of the colony, average diameter of the colony, and circularity of the colony. Refer to the manual section Changing Filter Settings.

Preview out of focus

If the preview image is out of focus, select Focus Preview from the Camera control module. If the camera is still out of focus select Calibrate & Lock Focus from the Camera control module.

No power to the cabinet

Recheck main power cord connections to the system and the wall power (surge protector).

Check the power switch on the back of the unit. Make sure it is in the I position.

Check the fuse located on the main power port. Replace if necessary. If the system continues to blow fuses, call UVP Technical Support Department.

Return Service Procedure

A Returned Goods Authorization (RGA) number must be obtained from UVP‟s Customer Service prior to returning any product.

        

All returns must be authorized by UVP whether for credit, warranty replacement or repair. Items returned for credit may be subject to a restocking charge.

Please contact our Customer Service Department for a Returned Goods Authorization (RGA).

No credit will be issued or allowed until UVP has had sufficient time to inspect the product and determine corrective action.

Returns must be made within 30 days of issuance of the RGA number and product must be in original packaging with all manuals and instructions.

RGA number is non-transferable and good for one use only.

Products returned for credit or replacement must be in like-new condition. All products must be free from Bio-Hazardous contamination with a contamination-free certificate attached. Contaminated products will be returned collect.

Products returned for repair will be evaluated by Quality Control. Estimated cost for repair will be submitted to customer for approval. If accepted, all evaluation costs will be credited towards entire repair. Non- repaired products can be returned at customer‟s cost.

Freight must be prepaid on goods returned to UVP by the customer.

For complete terms and conditions, contact UVP.

ColonyDoc-It Imaging Station

Technical Support

UVP offers expert technical support on all of our products. If there are any questions about the product‟s use, operation or repair, please contact our offices at the locations below.

If in North America, South America, East Asia or Australia:

Call (800) 452-6788 or (909) 946-3197, and ask for Technical Support during regular business days, between 7:00 am and 5:00 pm, PST. E-mail your message to: info@uvp.com or techsupport@uvp.com Fax Technical Support at (909) 946-3597

If in Europe, Africa, the Middle East or Western Asia:

Call +44(0) 1223-420022, and ask for

Customer Service during regular business days between 9:00 am and 5:30 pm. E-mail your message to: uvp@uvp.co.ukFax Customer Service at +44(0) 1223-420561

Write to: Ultra-Violet Products Ltd. Unit 1, Trinity Hall Farm Estate, Nuffield Road, Cambridge CB4 1TG UK

Write to: UVP, LLC 2066 W. 11Upland, CA 91786 USA

Street,

Warranty

UVP, LLC warrants all of its products (except tubes, grids and filters which is 90 days) to be free from defects in material and workmanship for a period of one (1) year from the date of purchase. All transilluminators carry a two (2) year warranty. The foregoing warranty of UVP shall be of no force and effect if the buyer has modified or damaged the product.

All warranties or merchantability and fitness for any purpose, and all other warranties, express or implied, except those expressly set forth herein, are deemed waived and excluded.

UVP's duty under the warranty is limited to replacement and/or repair of the defective part at the option of UVP, FOB, Upland, California. UVP shall not be liable for any expenses or damages incurred by purchaser except as expressly set forth herein, and in no event shall UVP be liable for any special, incidental or consequential damages of any kind.

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