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Ligation-based RNA amplification

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专利名称:Ligation-based RNA amplification发明人:Nelson, John R.,Grossman, Gregory

A.,Duthie, R. Scott,Sekher,Anuradha,Dhulipala, Ro-hini

申请号:AU2013203921申请日:20130411公开号:AU2013203921A1公开日:20130502

摘要:#$%^&*AU2013203921A120130502.pdf#####

H:\\scgIten.ve\\NRPonbl\\DCC'.SCG5064360_I.doc-I Ii4/21113 Abstract Methods ofamplification, purification and detection of nucleic acid sequences especially RNA aredescribed. One aspect of the method involves the hybridisation and subsequent ligationof a nucleic acid structure to the nucleic acid sequence desired to be manipulated. Themethods require that the nucleic acid structure comprises a double stranded region anda single stranded region. The single stranded region is complementary to the RNAsequence of interest. The double stranded region may also contain additionalfunctionalities which are then used subsequently in the method.

申请人:GE Healthcare Bio-Sciences Corp.

代理人:Davies Collison Cave

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